High-Dimensional mRNA and Protein Content Measurements in Single Cells with Single-Molecule Sensitivity
نویسندگان
چکیده
منابع مشابه
Simultaneous detection of mRNA and protein in single cells using immunofluorescence-combined single-molecule RNA FISH.
Although the concept of combining immunofluorescence (IF) with single-molecule RNA fluorescence in situ hybridization (smRNA FISH) seems obvious, the specific materials used during IF and smRNA FISH make it difficult to perform these procedures simultaneously on the same specimen. Even though there are reports where IF and smRNA FISH were combined with success, these were insufficient in terms ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2018
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.11.2911